Dendra2 fusions were transiently expressed in Nicotiana benthamiana leaf epidermal cells. The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara ⦠The transient expression of this mAb in wild-type N. benthamiana accumulated up to 344.12 ± 98.23 μg/g fresh leaf weight after 4 days of agroinfiltration. Here we use a transient expression system in the model plant Nicotiana benthamiana to reconstitute the two-step plant-derived biosynthetic pathway for auxin (indole acetic acid) to achieve accumulation up to 500 ng/g fresh mass (FM). Cultures were then diluted to an A 600 of 0.5 and injected into N. benthamiana leaves using a blunt-end 1 ml syringe. This high α-AIC3 accumulation allowed its structural and functional characterizations. 2022 May 13;13:912293. doi: 10.3389/fpls.2022.912293. Transient expression in tobacco plant is used to determine the subcellular location of a protein of interest when tagged with a reporter such as green fluorescent protein (GFP), or to mass produce proteins without making transgenic plants. Transient Expression of Homologous Hairpin RNA Interferes with ⦠Transient expression in Nicotiana benthamiana for rapid ⦠By expressing these plant-derived enzymes in combination with either bacterial halogenases and alternative substrates, we can ⦠Plant secondary metabolites have applications for the food, biofuel, and pharmaceutical industries.
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